Non-disruptive detection of DNA polymerases in nondenaturing polyacrylamide gels
نویسندگان
چکیده
منابع مشابه
Detection of DNA in polyacrylamide gels by autoradiography.
METHOD 1. Immerse the gel, together with its attached glass plate, in 7% acetic acid for 5 minutes. Remove the gel from the fixative by carefully lifting the glass plate from the fluid. 2. Rinse the gel briefly in deionized H2O. Remove excess fluid from the surface of the gel with a pad of Kimwipes. IMPORTANT Do not use absorbent paper; it will stick to the gel. 3. (Optional) Dry the gel onto a...
متن کاملComparison of various ultraviolet sources for fluorescent detection of ethidium bromide-DNA complexes in polyacrylamide gels.
Ultraviolet sources with output wavelengths or 254, 300, and 366 nm were compared for detection of ethidium bromide-DNA complexes in acrylamide gels. The 254and 300-nni sources were both much more sensitive than the 366-nni source. The 254-nni .source produced a great deal of photodamage, photonickingand photodimerization, and photobleaching, while the longer wavelength sources cause little dam...
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A method for the detection and quantitation of unlabeled nucleic acids in polyacrylamide gels is presented. The technique is a variation on UV shadowing. The technique is based on the observation that some storage phosphor screens (Kodak in this case) are sensitive to UV light in the range where nucleic acids absorb most strongly. Nucleic acid present in a gel resting on the screen absorbs the ...
متن کاملVisible fluorescent detection of proteins in polyacrylamide gels without staining.
2,2,2-Trichloroethanol (TCE) incorporated into polyacrylamide gels before polymerization provides fluorescent visible detection of proteins in less than 5min of total processing time. The tryptophans in proteins undergo an ultraviolet light-induced reaction with trihalocompounds to produce fluorescence in the visible range so that the protein bands can be visualized on a 300-nm transilluminator...
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Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10 moles of sulfite.
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1985
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1985.tb09102.x